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Updated: Jun 10, 2026

07:45
An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation
Published on: June 6, 2022
Fluorescent ratiometric sensing of pyrophosphate via induced aggregation of a conjugated polyelectrolyte
Xiaoyong Zhao1, Kirk S Schanze
1Department of Chemistry, University of Florida, Gainesville, FL, USA.
Abstract:
Direct detection of pyrophosphate (PPi) in aqueous solution is demonstrated using a cationic poly(phenylene-ethynylene) with polyamine side chains. Pyrophosphate-induced polymer aggregation causes a significant spectroscopic change, which in turn allows quantification of dissolved PPi using ratiometric signals.
