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Degradation of di-n-butyl phthalate by newly isolated Ochrobactrum sp
Xue-ling Wu1, Yang-yang Wang, Ren-xing Liang
1Department of Bioengineering, School of Minerals Processing and Bioengineering, Central South University, Changsha, Hunan Province, 410083, China. xueling0714@yahoo.com.cn
Abstract:
A gram negative isolate designated JDC-41 was obtained from river sludge using mixtures of phthalate esters as the sole source and energy. The isolate was identified as Ochrobactrum sp. based on its 16S rRNA gene sequence. Over 87% of supplied di-n-butyl phthalate (DBP) was degraded by JDC-41 in a pH neutral mineral salts medium at 30 degrees C within 48 h. Increased DBP (50-500 mg/L) in the culture correspondingly increased degradation half-life from 3.83 to 18.12 h. DBP induced cells more rapidly degraded DBP.
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