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Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing
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Selection of soluble protein expression constructs: the experimental determination of protein domain boundaries

Michael R Dyson1

  • 1Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QW, UK. md458@cam.ac.uk

Biochemical Society Transactions
|July 28, 2010
PubMed

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Conservation of Protein Domains Over Different Proteins02:26

Conservation of Protein Domains Over Different Proteins

Protein domains are small structurally independent units that are part of a single amino acid chain.  Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to form...