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Related Concept Videos

DNA Helicases00:55

DNA Helicases

DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
RNA Structure01:19

RNA Structure

The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
RNA Structure01:23

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...

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Related Experiment Video

Updated: Jun 10, 2026

A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1
11:25

A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1

Published on: March 18, 2017

Brr2p RNA helicase with a split personality: insights into structure and function.

Daniela Hahn1, Jean D Beggs

  • 1Wellcome Trust Centre for Cell Biology, University of Edinburgh, King's Buildings, Mayfield Road, Edinburgh EH9 3JR, Scotland, UK.

Biochemical Society Transactions
|July 28, 2010
PubMed
Summary

RNA helicases, like Brr2p, are crucial for RNA splicing. Recent studies reveal Brr2p's structure and function, linking its role in splicing to retinitis pigmentosa, a degenerative eye disease.

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Last Updated: Jun 10, 2026

A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1
11:25

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Published on: March 18, 2017

Visualization and Quantification of Intermolecular RNA Base Pairing in in vitro RNA Clusters Using Split Broccoli RNA Reporters
10:52

Visualization and Quantification of Intermolecular RNA Base Pairing in in vitro RNA Clusters Using Split Broccoli RNA Reporters

Published on: May 29, 2026

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • RNA helicases are essential for numerous cellular processes, including pre-mRNA splicing.
  • The DExD/H-box family of RNA helicases plays a critical role in spliceosome assembly and dynamics.
  • Brr2p, a spliceosomal RNA helicase, possesses a unique domain structure.

Purpose of the Study:

  • To review recent structural and biochemical findings on Brr2p.
  • To elucidate the mechanism and regulation of Brr2p activity.
  • To discuss the connection between human Brr2 function and retinitis pigmentosa.

Main Methods:

  • Structural biology studies (e.g., X-ray crystallography, cryo-EM).
  • Biochemical assays to assess RNA helicase activity.
  • Review of existing literature and genetic data.

Main Results:

  • Recent structural data provide insights into Brr2p's domain organization and function.
  • Biochemical studies illuminate the catalytic mechanisms and regulatory interactions of Brr2p.
  • Evidence suggests a link between Brr2p's splicing role and the pathology of retinitis pigmentosa.

Conclusions:

  • Advances in structural and biochemical analyses have significantly improved our understanding of Brr2p.
  • Brr2p's function in RNA splicing is implicated in the pathogenesis of retinitis pigmentosa.
  • Further research may reveal therapeutic strategies targeting Brr2p in eye diseases.