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Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis
Published on: June 3, 2016
Transcription factor activator protein-2beta accelerates lipid accumulation in macrophages via enhancing the
Lu Gan1, Dongxing Zhu, Zhenhua Ding
1Department of Radiation Medicine, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, Guangdong 510515, China. gan_lu@yahoo.com.cn
Abstract:
The role of transcription factor activator protein-2beta (AP-2beta) in foam cell formation was investigated. Uptake assay of DiI-labeled oxidized low density lipoprotein and the lipid quantitative analysis by high performance liquid chromatography showed AP-2beta promoted the lipid accumulation. The quantitative real-time RT-PCR and Western blot indicated that AP-2beta upregulated the expression of CD36. Luciferase assay, electrophoretic mobility shift assay and chromatin immunoprecipitation confirmed that AP-2beta bound to AP-2 binding sites in CD36 promoter region and enhanced the promoter activity of CD36 gene. We conclude that AP-2beta may function in foam cell formation through enhancing the transcription and expression of CD36 followed by increased lipid uptake.
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