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Related Concept Videos

Spectrophotometry: Introduction01:16

Spectrophotometry: Introduction

Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
IR Spectrometers01:25

IR Spectrometers

There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...
UV–Vis Spectrometers01:14

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...
Instrument Calibration01:12

Instrument Calibration

Instrument calibration is essential for ensuring that instruments produce accurate and consistent results. It is vital in manufacturing, healthcare, testing laboratories, and scientific research. Calibration processes are specific to each instrument and help enhance data accuracy. Each instrument has a unique calibration process tailored to its design and function to improve data accuracy.
Analytical Balance Calibration
An analytical balance measures mass and requires regular calibration to...
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
Electronic Distance Measuring Instruments01:30

Electronic Distance Measuring Instruments

Electronic Distance Measuring Instruments (EDMs) are essential tools in modern surveying, offering precise distance measurements by emitting electromagnetic signals and calculating the time required for these signals to travel to a target and return. Two primary types of signals are used in EDMs — light waves and microwaves — each suited to specific environmental and distance requirements. Light-wave-based EDMs utilize either infrared or laser light, providing high accuracy over short distances...

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Related Experiment Video

Updated: Jun 10, 2026

Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores
09:46

Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores

Published on: August 19, 2013

Four color-measuring devices compared with a spectrophotometric reference system.

Karl Martin Lehmann1, Christopher Igiel, Irene Schmidtmann

  • 1Clinic of Prosthetic Dentistry, University Medical Center of the Johannes Gutenberg-University of Mainz, Augustusplatz 2, Mainz, Germany. lehmann@prothetik.klinik.uni-mainz.de

Journal of Dentistry
|July 28, 2010
PubMed
Summary
This summary is machine-generated.

Four dental color-measuring devices were evaluated against a spectrophotometric reference system. Devices C and D demonstrated higher precision and accuracy in L*C*h° color coordinate measurements compared to devices A and B.

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Measurement of Carotenoids in Perifovea using the Macular Pigment Reflectometer
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Last Updated: Jun 10, 2026

Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores
09:46

Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores

Published on: August 19, 2013

Measurement of Carotenoids in Perifovea using the Macular Pigment Reflectometer
09:35

Measurement of Carotenoids in Perifovea using the Macular Pigment Reflectometer

Published on: January 29, 2020

Area of Science:

  • Dental materials science
  • Colorimetry
  • Optical measurements

Background:

  • Accurate shade matching is crucial in restorative dentistry.
  • Electronic dental color-measuring devices aim to improve shade selection accuracy.
  • Comparison with a reference spectrophotometric system is essential for device validation.

Purpose of the Study:

  • To compare L*C*h° color coordinates from four dental color-measuring devices with a spectrophotometric reference system.
  • To evaluate the accuracy and precision of electronic dental shade guides.

Main Methods:

  • Four devices (VITA Easyshade, VITA Easyshade compact, Degudent Shadepilot, X-Rite Shadevision) were tested.
  • L*C*h° color coordinates of ceramic samples were recorded under standardized conditions.
  • Intraclass correlation coefficients, regression analysis, and mean deviations were calculated.

Main Results:

  • All devices exhibited high intraclass correlation coefficients (0.979-1.000).
  • Devices C and D showed regression slopes and intercepts closer to optimal values.
  • Devices C and D demonstrated higher precision compared to devices A and B.

Conclusions:

  • Electronic dental color-measuring devices show excellent repeatability.
  • Some devices exhibit significant deviations from spectrophotometric reference values.
  • Degudent Shadepilot and X-Rite Shadevision offer superior precision for dental color measurement.