[Effect of nucleotide anti-aggregants (NAD, AMP) and ischemia on the tissue blood coagulation factors]

It was shown in experiments on white rats that combined administration of nucleotide antiaggregants NAD+ and AMP, and NAD+ decomposition inhibitor--nicotinamide, decreased the increment of procoagulant (thromboplastic) activity of membrane particles in the ischemized kidneys and reduced thromboplastic activity of membrane particles in intact kidneys. When nucleotide antiaggregants were added to rabbit citrate plasma, the inhibition by membrane activation of recalcified plasma coagulation was more pronounced with the use of tissue thromboplastin of the kidneys and brain--proteophospholipid liposomes, than with the use of phospholipid liposomes obtained from thromboplastin. The data presented have evidenced an indirect anticoagulant effect of nucleotide aggregants at the level of tissue coagulation factors, and their direct anticoagulant action at the level of membrane activation of plasma factors.