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Putrescine production by engineered Corynebacterium glutamicum.

Jens Schneider1, Volker F Wendisch

  • 1Genetics of Prokaryotes, Faculty of Biology & CeBiTec, University of Bielefeld, PO Box 100131, 33501 Bielefeld, Germany.

Applied Microbiology and Biotechnology
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Engineered Corynebacterium glutamicum to produce putrescine, achieving 6 g/L. Optimized the ornithine decarboxylase pathway for higher production efficiency, demonstrating its industrial potential.

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Area of Science:

  • Biotechnology and metabolic engineering
  • Microbial strain development
  • Industrial microbiology

Background:

  • Corynebacterium glutamicum is a key industrial microorganism.
  • Putrescine is a valuable bioproduction precursor.
  • Engineering C. glutamicum for enhanced putrescine production is of significant interest.

Purpose of the Study:

  • To engineer Corynebacterium glutamicum for efficient putrescine production.
  • To compare the efficacy of arginine decarboxylase and ornithine decarboxylase pathways for putrescine synthesis.
  • To optimize the metabolic pathways for maximizing putrescine yield and productivity.

Main Methods:

  • Heterologous expression of Escherichia coli arginine and ornithine decarboxylase pathway genes in C. glutamicum.
  • Genetic modifications including gene overexpression (speC) and gene deletions (ArgR, ArgF).
  • Shake-flask batch cultivation to assess putrescine production, yield, and productivity.

Main Results:

  • C. glutamicum tolerated putrescine up to 500 mM, with growth rate and biomass affected at 750 mM.
  • The ornithine decarboxylase pathway resulted in a 40-fold higher putrescine yield compared to the arginine decarboxylase pathway.
  • The optimized strain produced up to 6 g/L putrescine with a space-time yield of 0.1 g/L/h and an overall yield of 24 mol%.

Conclusions:

  • Engineering C. glutamicum via the ornithine decarboxylase pathway is a viable strategy for industrial putrescine production.
  • Overexpression of speC and deletion of ArgR and ArgF significantly enhanced putrescine production efficiency.
  • The developed strain shows promise for the cost-effective biosynthesis of putrescine.