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Related Experiment Video

Updated: Jun 10, 2026

A Tandem Liquid Chromatography&#8211;Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus
08:03

A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus

Published on: March 28, 2017

Profiling the surfacome of Staphylococcus aureus.

Annette Dreisbach1, Kristina Hempel, Girbe Buist

  • 1Department of Medical Microbiology, University Medical Centre Groningen and University of Groningen, Groningen, The Netherlands.

Proteomics
|July 28, 2010
PubMed
Summary

Staphylococcus aureus surface proteins, or the surfacome, are crucial for infection. This study reveals significant strain-specific variations in the S. aureus surfacome, highlighting its heterogeneity.

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Area of Science:

  • Microbiology
  • Proteomics
  • Pathogen Biology

Background:

  • Staphylococcus aureus is a major human pathogen responsible for diverse infections.
  • Surface-exposed proteins are critical for S. aureus colonization and virulence.
  • Understanding the S. aureus surfacome is key to developing novel therapeutic strategies.

Purpose of the Study:

  • To optimize a proteolytic shaving method for identifying surface-exposed proteins (surfacome) of S. aureus.
  • To characterize and compare the surfacomes of four genetically distinct S. aureus strains.
  • To investigate the variability and heterogeneity of the S. aureus cell surface proteome.

Main Methods:

  • Optimization of a proteolytic shaving technique to isolate surface proteins.
  • Application of the optimized method to four different S. aureus strains.
  • Proteomic analysis of identified surface proteins and peptides using mass spectrometry.

Main Results:

  • Identification of 96 distinct surface-exposed proteins across four S. aureus strains.
  • Low overlap (<10%) in surfacomes between different strains, indicating strain-specific protein expression.
  • High variability observed at both protein and peptide levels, with only 5 common peptides found among all strains.
  • Inclusion of known cell wall proteins, essential proteins, uncharacterized exported proteins, and predicted intracellular proteins in the surfacome.

Conclusions:

  • The cell surface proteome of S. aureus is highly variable and strain-dependent.
  • Significant heterogeneity exists in the displayed surface proteins among different S. aureus strains.
  • These findings provide new insights into S. aureus surface protein diversity and its implications for pathogenesis.