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Published on: March 16, 2013
Effect of endothelial nitric oxide synthase on epidermal permeability barrier recovery after disruption
1Shiseido Innovative Science Research & Development Center, 2-12-1 Fukuura, Kanazawa-ku, Yokohama 236-8643, Japan. kazuyuki.ikeyama@to.shiseido.co.jp
Background:
We previously demonstrated that neuronal nitric oxide synthase (nNOS) in epidermal keratinocytes is associated with epidermal permeability barrier homeostasis.
Objectives:
In the present study, we examined the contributions of inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS) to epidermal permeability barrier homeostasis.
Methods:
We measured the barrier recovery rate after tape stripping of the epidermis of iNOS and eNOS knockout mice, and carried out electron-microscopic observation of the epidermis after acetone treatment.
Results:
The barrier recovery rate of eNOS knockout mice was significantly faster than that of the wild-type control, while no significant difference was observed between iNOS knockout mice and wild-type mice. Electron-microscopic observation at 1 h after acetone treatment indicated that barrier recovery of both nNOS and eNOS mice was faster than that of wild-type mice, and lamellar body secretion was accelerated in both types of knockout mice.
Conclusions:
These results suggested that both nNOS and eNOS play roles in epidermal barrier homeostasis and lamellar body secretion.
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