Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Electrical responses from the chicken basilar papilla.

R B Patuzzi1, C L Bull

  • 1Physiology Department, University of Western Australia, Nedlands.

Hearing Research
|May 1, 1991
PubMed
Summary

Researchers developed a surgical method to record from chicken cochlear hair cells in vivo. They found that these hair cells are not electrically tuned, with tuning solely driven by mechanical forces on their hair bundles.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A structural study of PrCrO<sub>3</sub> under extreme conditions: a comparison with the effects of doping.

Philosophical transactions. Series A, Mathematical, physical, and engineering sciences·2023
Same author

Ostwald's rule of stages and metastable transitions in the hydrogen-water system at high pressure.

Physical chemistry chemical physics : PCCP·2018
Same author

Magnetic and structural changes in LaCo<sub>0.9</sub>Mn<sub>0.1</sub>O<sub>3</sub> at high pressure.

Journal of physics. Condensed matter : an Institute of Physics journal·2017
Same author

Probing ice VII crystallization from amorphous NaCl-D<sub>2</sub>O solutions at gigapascal pressures.

Physical chemistry chemical physics : PCCP·2016
Same author

Structural characterization of eutectic aqueous NaCl solutions under variable temperature and pressure conditions.

Physical chemistry chemical physics : PCCP·2015
Same author

Urea and deuterium mixtures at high pressures.

The Journal of chemical physics·2015

Area of Science:

  • Oto-neurology
  • Auditory Neuroscience
  • Cellular Electrophysiology

Background:

  • The basilar papilla in the chicken cochlea is crucial for auditory processing.
  • Understanding the electrophysiological properties of hair cells is key to understanding hearing mechanisms.
  • Previous studies suggested potential electrical tuning in hair cells, but in vivo data was limited.

Purpose of the Study:

  • To develop a surgical technique for in vivo recordings within chicken cochlear hair cells and supporting cells.
  • To investigate the electrophysiological properties and frequency tuning of these cells.
  • To determine if chicken cochlear hair cells exhibit electrical tuning.

Main Methods:

  • Developed a surgical approach for in vivo recordings in the chicken basilar papilla.
  • Measured AC receptor potentials and membrane properties using microelectrodes within hair cells and supporting cells.
  • Utilized fluorescent dye-marking and electrode angle analysis to identify impaled cell types.
  • Performed current injection to assess cell-voltage relationships.

Main Results:

  • Frequency tuning curves for AC receptor potentials showed best frequencies from 600 Hz to 2000 Hz.
  • No spontaneous oscillations or ringing were observed in hair cell membrane potentials.
  • Hair cell membrane potential was linearly modulated by papilla displacement, indicating a linear current-voltage relationship.
  • Evidence suggests the impaled cells were short hair cells, not electrically tuned under experimental conditions.

Conclusions:

  • Chicken cochlear hair cells, specifically short hair cells, are not electrically tuned in vivo.
  • The observed frequency tuning of AC receptor potentials is attributed to mechanical drive to the hair bundles.
  • This study provides crucial in vivo electrophysiological data on avian auditory hair cells.

Related Experiment Videos