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Updated: Oct 21, 2026
11:19
Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System
Published on: August 21, 2016
The hemimethylated replication origin of Escherichia coli can be initiated in vitro
1Department of Biophysics, Norwegian Radium Hospital, Oslo.
Journal of Bacteriology
|July 1, 1991
Abstract:
Unmethylated, fully methylated, and hemimethylated oriC-containing plasmids were assayed as substrates for DNA replication in vitro by using a system reconstituted with pure proteins. In contrast to the in vivo situation, all three substrates were initiated efficiently; the fully methylated plasmid was about twice as active as the other two.
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