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DNA-bridged bioconjugation of fluorescent quantum dots for highly sensitive microfluidic protein chips

Mei Hu1, Yao He, Shiping Song

  • 1Laboratory of Advanced Materials, School of Life Sciences, Fudan University, 220 Handan Road, Shanghai 200433, China.

Chemical Communications (Cambridge, England)
|July 29, 2010
PubMed

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...