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Updated: Jun 10, 2026

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
The complete nucleotide sequence of an isolate of Tomato yellow leaf curl Sardinia virus found in Sicily
Salvatore Davino1, Laura Miozzi, Gian Paolo Accotto
1Dipartimento di Scienze Entomologiche, Fitopatologiche, Microbiologiche e Zootecniche, Università degli Studi di Palermo, Viale delle Scienze, Palermo, Italy.
Abstract:
Partial sequences of Tomato yellow leaf curl Sardinia virus (TYLCSV) derived from tomato samples collected in Sicily in 1999, 2002 and 2004 indicated the presence of a TYLCSV different from the one previously described as the Sic strain. Here, we report a complete DNA sequence that is classified as belonging to the TYLCSV type strain (Sar strain), confirming the co-existence in Sicily of virus populations of both strains. Moreover, comparisons between this new sequence and those of the two recombinants recently described in Sicily revealed unequivocally (99% identity) that their TYLCSV-derived portion originated from the Sar strain.
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