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Physical maps for HSV type 2 DNA with five restriction endonucleases.

R Cortini, N M Wilkie

    The Journal of General Virology
    |May 1, 1978
    PubMed
    Summary
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    Researchers mapped Herpes Simplex Virus 2 (HSV-2) DNA using molecular hybridization. The constructed physical maps show HSV-2 genome regions are similar in molecular weight to HSV-1.

    Area of Science:

    • Molecular Biology
    • Virology
    • Genomics

    Background:

    • Herpes Simplex Virus 2 (HSV-2) is a significant human pathogen.
    • Understanding the HSV-2 genome structure is crucial for virological research.
    • Previous studies have established physical maps for HSV-1, providing a basis for comparison.

    Purpose of the Study:

    • To construct a physical map of the Herpes Simplex Virus 2 (HSV-2) genome.
    • To determine the ordering of restriction endonuclease fragments of HSV-2 DNA.
    • To compare the molecular weights of HSV-2 genome regions with those of HSV-1.

    Main Methods:

    • Utilized molecular hybridization techniques to analyze HSV-2 DNA fragments.
    • Employed cleavage of isolated restriction endonuclease fragments with further restriction endonucleases.

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  • Constructed physical maps using restriction enzymes EcoRI, Hind III, Bgl II, Xba, and Hpa I.

    • Main Results:

    • Successfully constructed physical maps for HSV-2 DNA fragments generated by specified restriction enzymes.
    • Determined the precise ordering of these fragments within the HSV-2 genome.
    • Found that the molecular weights of HSV-2 genome regions are highly similar to corresponding regions in the HSV-1 genome.

    Conclusions:

    • The study provides a detailed physical map of the HSV-2 genome.
    • The findings highlight significant structural similarities between HSV-1 and HSV-2 genomes.
    • This research contributes to a better understanding of herpesvirus genome organization and evolution.