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Updated: Jun 10, 2026
10:50
Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System
Published on: May 1, 2019
Development of fluoroapatite chromatography for the purification of monoclonal antibody
Paul K Ng1, Tomohiko Yoshitake, Mark A Snyder
1Process R&D Applications, Life Science Group, Bio-Rad Laboratories, Hercules, CA 94547, USA. paul_ng@bio-rad.com
Journal of Separation Science
|July 29, 2010
Abstract:
Monoclonal antibody was purified from tissue culture fluid using ceramic fluoroapatite chromatography. Efficiency of the capture step was shown to be sensitive to pH and phosphate concentration. More than 90% of host cell proteins were removed by ceramic fluoroapatite chromatography. Studies regarding pH control and metal adsorption are presented.
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