Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...
Stem Cell Culture01:17

Stem Cell Culture

Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
iPS Cell Differentiation01:22

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
EPS and iPS Cells in Disease Research01:21

EPS and iPS Cells in Disease Research

Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Alternative AAV gene therapy for hemophilia A using expression of Bi8, a novel single-chain FVIII-mimetic antibody.

Blood·2025
Same author

AAV Gene Therapy in Severe Hemophilia B. Reply.

The New England journal of medicine·2025
Same author

Sustained Clinical Benefit of AAV Gene Therapy in Severe Hemophilia B.

The New England journal of medicine·2025
Same author

FLT201, a novel liver-directed AAV gene therapy candidate for Gaucher disease type 1.

Molecular therapy : the journal of the American Society of Gene Therapy·2025
Same author

Expression and treatment of ROR1+ cells with bispecific T-cell engagers in pediatric acute lymphoblastic leukemia.

Blood advances·2025
Same author

10 years of BiTE immunotherapy: an overview with a focus on pancreatic cancer.

Frontiers in oncology·2025

Related Experiment Video

Updated: Jun 10, 2026

Generation of Induced Pluripotent Stem Cells from Frozen Buffy Coats using Non-integrating Episomal Plasmids
10:52

Generation of Induced Pluripotent Stem Cells from Frozen Buffy Coats using Non-integrating Episomal Plasmids

Published on: June 5, 2015

An introduction to induced pluripotent stem cells.

Joanna Hanley1, Ghasem Rastegarlari, Amit C Nathwani

  • 1Department of Haematology, UCL Cancer Institute, London, UK.

British Journal of Haematology
|July 30, 2010
PubMed
Summary

Induced pluripotent stem (iPS) cells offer a promising alternative to human embryonic stem (hES) cells, derived from patients to avoid immune rejection. Their immediate applications are in drug screening and disease research, though clinical use faces challenges.

More Related Videos

Selecting and Isolating Colonies of Human Induced Pluripotent Stem Cells Reprogrammed from Adult Fibroblasts
13:23

Selecting and Isolating Colonies of Human Induced Pluripotent Stem Cells Reprogrammed from Adult Fibroblasts

Published on: February 20, 2012

Isolation of Adult Human Dermal Fibroblasts from Abdominal Skin and Generation of Induced Pluripotent Stem Cells Using a Non-Integrating Method
10:52

Isolation of Adult Human Dermal Fibroblasts from Abdominal Skin and Generation of Induced Pluripotent Stem Cells Using a Non-Integrating Method

Published on: January 19, 2020

Related Experiment Videos

Last Updated: Jun 10, 2026

Generation of Induced Pluripotent Stem Cells from Frozen Buffy Coats using Non-integrating Episomal Plasmids
10:52

Generation of Induced Pluripotent Stem Cells from Frozen Buffy Coats using Non-integrating Episomal Plasmids

Published on: June 5, 2015

Selecting and Isolating Colonies of Human Induced Pluripotent Stem Cells Reprogrammed from Adult Fibroblasts
13:23

Selecting and Isolating Colonies of Human Induced Pluripotent Stem Cells Reprogrammed from Adult Fibroblasts

Published on: February 20, 2012

Isolation of Adult Human Dermal Fibroblasts from Abdominal Skin and Generation of Induced Pluripotent Stem Cells Using a Non-Integrating Method
10:52

Isolation of Adult Human Dermal Fibroblasts from Abdominal Skin and Generation of Induced Pluripotent Stem Cells Using a Non-Integrating Method

Published on: January 19, 2020

Area of Science:

  • Stem cell biology
  • Regenerative medicine
  • Cellular reprogramming

Background:

  • Human embryonic stem (hES) cells face ethical limitations and immunological rejection issues.
  • Induced pluripotent stem (iPS) cells offer a potential solution by bypassing these concerns.
  • Somatic cells can be reprogrammed into iPS cells, resembling embryonic stem cells.

Purpose of the Study:

  • To review the potential of iPS cells in clinical applications.
  • To discuss the challenges associated with the clinical use of iPS cells.
  • To highlight the advantages of patient-specific iPS cells.

Main Methods:

  • Reprogramming of somatic cells (fibroblasts, lymphocytes) into iPS cells.
  • Generation of patient-specific iPS cells to avoid immune rejection.
  • Comparison of iPS cells with human embryonic stem cells.

Main Results:

  • iPS cells can be generated without human embryos or oocytes.
  • Patient-derived iPS cells overcome immunological rejection.
  • Immediate utility of iPS cells lies in drug screening and disease modeling.

Conclusions:

  • iPS cells present significant clinical potential.
  • Challenges remain for the widespread clinical application of iPS cells.
  • Further research is needed to overcome these challenges for therapeutic use.