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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Updated: Jun 10, 2026

Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution

Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution

Published on: May 12, 2023

Digital genome-wide ncRNA expression, including SnoRNAs, across 11 human tissues using polyA-neutral amplification.

John C Castle¹, Christopher D Armour, Martin Löwer

¹Institute for Translational Oncology and Immunology, Mainz, Germany. castle@uni-mainz.de

|July 30, 2010

Summary

This summary is machine-generated.

This study reveals high expression levels of non-coding RNAs (ncRNAs) across human tissues using a novel sequencing method. These findings highlight the diverse roles and tissue-specific patterns of ncRNAs, crucial for molecular biology research.

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Chromatin Isolation by RNA Purification (ChIRP)

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Chromatin Isolation by RNA Purification (ChIRP)

Chromatin Isolation by RNA Purification (ChIRP)

Published on: March 25, 2012

Area of Science:

Molecular Biology
Genomics
Biochemistry

Background:

Non-coding RNAs (ncRNAs) are vital but challenging to study due to lack of polyadenylation.
High-throughput monitoring of ncRNAs requires specialized techniques.

Purpose of the Study:

To explore ncRNA expression across eleven human tissues using a polyadenylation-neutral amplification protocol.
To characterize the expression patterns and abundance of various ncRNA classes.

Main Methods:

Polyadenylation-neutral amplification protocol.
Next-generation sequencing (NGS).
Analysis of ncRNA expression in eleven human tissues.

Main Results:

ncRNAs like 7SL, U2, 7SK, and HBII-52 show higher expression than messenger RNAs (mRNAs).
Specific expression patterns observed for C/D and H/ACA box snoRNAs in spleen, hypothalamus, and testes.
Enriched expression of mitochondrial tRNAs and novel transcription in ovaries within Prader-Willi syndrome loci.

Conclusions:

ncRNA expression is rich, abundant, and exhibits function- and tissue-specific patterns.
The study provides a valuable genome-wide compendium of ncRNA expression.
This data is publicly available for further research.