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Updated: Jun 10, 2026

Assessment of Immunologically Relevant Dynamic Tertiary Structural Features of the HIV-1 V3 Loop Crown R2 Sequence by ab initio Folding
Published on: September 15, 2010
A novel SR protein binding site in a cis-regulatory element of HIV-1
Ornpreya Suptawiwat1, Chompunuch Boonarkart, Prasert Auewarakul
1Department of Microbiology, Siriraj Hospital, Mahidol University, Bangkok, Thailand. ornpreya@yahoo.com
Abstract:
We have previously described a cis enhancing sequence (CES) in the gp41 region of the HIV-1 env gene. It could enhance HIV-1 Gag expression via both rev-dependent and CTE (constitutive transport element)-dependent pathways. We identified a functionally important and conserved region in the CES that contained a predicted binding sequence for an SR protein, ASF/SF2. We show here that ASF/SF2 bound to this sequence in an electrophoretic mobility shift assay and that the putative ASF/SF2-binding sequence was required for the enhancement of Gag expression by CES and might play a role in HIV-1 posttranscriptional regulation.
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