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Updated: Jun 10, 2026
08:10
Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
Published on: August 8, 2016
Continuous screening system for inhibited enzyme catalysis: a membrane reactor approach.
Evgenij Lyagin1, Anja Drews, Subhamoy Bhattacharya
1Technische Universität Berlin, Chair of Chemical and Process Engineering, Berlin, Germany. evgenij.lyagin@tu-berlin.de
Biotechnology Journal
|July 30, 2010
Summary
This study introduces a novel continuous screening system for bioprocess development, overcoming limitations of batch methods to accurately assess catalysts and conditions.
Area of Science:
- Biotechnology
- Bioprocess Engineering
- Catalysis Research
Background:
- Bioprocess development demands extensive, costly, and time-consuming experimental screening.
- Current batch/fed-batch screening systems risk misinterpreting catalyst performance due to substrate inhibition or product accumulation.
- Essential properties like turnover stability can be masked by product inhibition in conventional systems.
Purpose of the Study:
- To develop an innovative screening system enabling continuous substrate feeding and product removal.
- To address the limitations of existing bioprocess screening methodologies.
- To improve the accuracy and efficiency of early-stage bioprocess development.
Main Methods:
- Design and operation of a prototype screening system.
- Utilizing a membrane reactor concept for continuous processing.
- Application to a model reaction: cellulose hydrolysis.
Main Results:
- Demonstration of a functional prototype for continuous bioprocess screening.
- Successful operation for the cellulose hydrolysis model reaction.
- Potential to overcome limitations of batch screening systems.
Conclusions:
- The developed continuous screening system offers a more accurate assessment of catalysts and conditions.
- This novel approach mitigates misinterpretations caused by substrate or product inhibition.
- The membrane reactor-based system represents a significant advancement in bioprocess development tools.
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