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Updated: Jun 10, 2026
08:20
Using Microarrays to Interrogate Microenvironmental Impact on Cellular Phenotypes in Cancer
Published on: May 21, 2019
[A verification study on the genes associated with laryngeal squamous cell carcinoma by cDNA microarray]
Xiaohui Shen1, Rongsheng Ni, Xiaoyun Qian
1Department of Otorhinolaryngology-Head and Neck Surgery, Drum Tower Hospital, School of Medicine, Nanjing University, Nanjing, 210008, China.
Summary
SENP1, CD109, Laminin alpha 3 mRNA and protein expression are elevated in laryngeal squamous cell carcinoma (LSCC), while Laminin alpha 2 mRNA is decreased. These findings suggest potential roles in LSCC development.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Previous cDNA microarray identified differentially expressed genes in laryngeal squamous cell carcinoma (LSCC).
- Understanding gene expression alterations is crucial for LSCC pathogenesis research.
Purpose of the Study:
- To validate the differential expression of specific genes (SENP1, CD109, Laminin alpha 2, Laminin alpha 3) in LSCC.
- To investigate the potential correlation of these genes with LSCC tumorigenesis and development.
Main Methods:
- Selected genes: SENP1, CD109, Laminin alpha 2, Laminin alpha 3 based on microarray data.
- Quantitative analysis of mRNA expression using RT-PCR.
- Assessment of protein expression via Western blot.
- Study included 12 cases of LSCC and adjacent normal tissues.
Main Results:
- SENP1, CD109, and Laminin alpha 3 mRNA levels were significantly higher in LSCC tissues compared to normal tissues.
- Laminin alpha 2 mRNA levels were significantly lower in LSCC tissues.
- SENP1 and CD109 protein expression were significantly elevated in LSCC tissues.
Conclusions:
- SENP1, CD109, Laminin alpha 2, and Laminin alpha 3 show altered expression patterns in LSCC.
- These genes may play a role in the tumorigenesis and progression of LSCC.
- Findings provide molecular insights into LSCC pathogenesis.
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