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Bioconversion of silver salt into silver nanoparticles using different microorganisms.

Sourish Karmakar1, Subir Kundu, Kanika Kundu

  • 1School of Biochemical Engineering, Institute of Technology, Banaras Hindu University, Varanasi, India.

Artificial Cells, Blood Substitutes, and Immobilization Biotechnology
|July 31, 2010
PubMed
Summary
This summary is machine-generated.

This study explored microbial synthesis of silver nanoparticles (AgNPs) using Aspergillus flavus, Phoma exigua, and Bacillus megaterium. Aspergillus flavus demonstrated the most effective conversion of silver nitrate into stable aqueous silver nanoparticles.

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Area of Science:

  • Biotechnology
  • Nanotechnology
  • Microbiology

Background:

  • Chemical synthesis of nanoparticles poses environmental risks due to pollutant release.
  • Biochemical approaches for nanoparticle synthesis are gaining traction as a sustainable alternative.
  • Silver nanoparticles (AgNPs) have diverse applications, driving interest in efficient production methods.

Purpose of the Study:

  • To investigate the potential of three microorganisms (Aspergillus flavus, Phoma exigua, Bacillus megaterium) for biosynthesis of silver nanoparticles.
  • To compare the efficacy of these microorganisms in reducing silver nitrate to AgNPs.
  • To characterize the synthesized AgNPs and assess microbial growth in the presence of silver nitrate.

Main Methods:

  • Incubation of microbial biomass (Aspergillus flavus, Phoma exigua, Bacillus megaterium) with silver nitrate solution for 48 hours.
  • Analysis of nanoparticle properties using surface plasmon resonance and Transmission Electron Microscopy (TEM).
  • Assessment of microbial growth in media containing silver nitrate.

Main Results:

  • Phoma exigua exhibited the highest reducing capacity initially, but Aspergillus flavus proved most effective overall for aqueous AgNP production.
  • Synthesized nanoparticles displayed characteristic optical and physical properties.
  • Fungal species showed varied growth in silver nitrate; bacterial species (Bacillus megaterium) did not grow.

Conclusions:

  • Aspergillus flavus is the most effective microorganism for the biosynthesis of stable aqueous silver nanoparticles from silver nitrate.
  • Fungal species can be directly used for AgNP production by incubating their inocula in silver nitrate-containing media.
  • Microbial synthesis offers a promising eco-friendly route for producing silver nanoparticles.