Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Mismatch Repair01:20

Mismatch Repair

Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
Mismatch Repair01:36

Mismatch Repair

Overview
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
Gene Conversion02:08

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Microstructural Properties and Pressure Distribution in Ultra-Short-Pulse Welds of Sapphire to Iron.

Nanomaterials (Basel, Switzerland)·2026
Same author

Population-scale chemical response revealed by a barcoded yeast collection.

Nature communications·2026
Same author

Domestication drives repeated evolution of sexual-asexual life cycle trade-offs in yeast.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Dynamics of genome evolution in the era of pangenome analysis.

Cell genomics·2025
Same author

From genotype to phenotype with 1,086 near telomere-to-telomere yeast genomes.

Nature·2025
Same author

A genetic screen reveals a key role for Reg1 in 2-deoxyglucose sensing and yeast AMPK inhibition.

PLoS genetics·2025

Related Experiment Video

Updated: Jun 10, 2026

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
07:55

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

Published on: September 11, 2022

Single-stranded heteroduplex intermediates in lambda Red homologous recombination.

Marcello Maresca1, Axel Erler, Jun Fu

  • 1Technische Universität Dresden, BioInnovationsZentrum, Dresden, Germany.

BMC Molecular Biology
|July 31, 2010
PubMed
Summary

Lambda phage Red proteins facilitate efficient homologous recombination. This study reveals a new

More Related Videos

Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy
11:40

Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy

Published on: June 25, 2013

Related Experiment Videos

Last Updated: Jun 10, 2026

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
07:55

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

Published on: September 11, 2022

Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy
11:40

Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy

Published on: June 25, 2013

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Lambda phage Red proteins mediate highly efficient homologous recombination.
  • The precise mechanism of double-stranded DNA (dsDNA) recombination by Red proteins is not fully understood.

Purpose of the Study:

  • To elucidate the mechanism of dsDNA recombination mediated by lambda phage Red proteins.
  • To propose and validate a new model for Red-mediated dsDNA recombination.

Main Methods:

  • Utilized asymmetrically digestible dsDNA substrates exploiting Redalpha exonuclease activity.
  • Investigated recombination efficiency based on homology region configuration and replication.
  • Employed an in situ assay to assess recombination outcomes and sensitivity to non-homologies.

Main Results:

  • Red proteins can operate through single-stranded (ssDNA) intermediates, forming ssDNA heteroduplexes at the replication fork.
  • Optimal dsDNA recombination occurs when the priming strand contains both homology regions.
  • Red-mediated recombination necessitates target molecule replication.
  • Recombination efficiency is more sensitive to 5' than 3' end non-homologies.

Conclusions:

  • A novel dsDNA recombination model, 'beta' recombination, is proposed, involving ssDNA heteroduplexes at the replication fork.
  • Beta recombination efficiently generates large deletions but is size-sensitive for insertions.
  • Findings offer practical insights into recombineering using Red proteins.