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RACE - Rapid Amplification of cDNA Ends

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
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Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization

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Data Collection Order: A Primer

T Deshefy-Longhi¹, S Sullivan-Bolyai, J K Dixon

¹DNSc, RN Postdoctoral Fellow Duke University Center for Aging and Human Development and School of Nursing Durham, North Carolina.

Southern Online Journal of Nursing Research

|July 31, 2010

Summary

No abstract available in PubMed .

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