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Sequence-specific primers for MNS blood group genotyping

Guido A Heymann¹, Abdulgabar Salama

¹Central Department of Laboratory Medicine, DRK-Kliniken-Berlin, USA. g.heymann@drk-kliniken-berlin.de

Blood Transfusion = Trasfusione Del Sangue

|July 31, 2010

Abstract

Background:

Various techniques of genotyping the MNSs blood group have been described, but none of them enables the complete detection of all MNS antigens.

Materials And Methods:

Blood samples were obtained from blood donors. Primers were created using the published DNA sequences for glycophorins A and B. Genotyping was performed using polymerase chain reaction sequence-specific primers (PCR-SSP).

Results:

A total of seven primers were found to specifically amplify the most common MNS antigens. The use of these primers has enabled us to correctly genotype all blood samples tested so far (n=116).

Discussion:

Specifically created primers enable genotyping of the MNS antigens in a single PCR-SSP run. The method is reliable, easy to perform, and can be used in routine practice.

Keywords:

MNS blood group PCR glycophorin

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Blood Types

Blood Types

Human blood is classified into different types based on the presence of antigens on the red blood cell's surface and antibodies in the plasma. Proper identification of blood type is essential for successful blood transfusion. The International Society of Blood Transfusion has identified 38 human blood types based on the surface antigens on the red blood cells. The most common types are ABO, Rh, and MNS blood types.
ABO blood group
ABO antigens are glycoproteins encoded by genes present on...

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