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Updated: Aug 8, 2026
Induction and Analysis of Epithelial to Mesenchymal Transition
Published on: August 27, 2013
[Research of TGF-beta1 inducing lung adencarcinoma PC9 cells to mesenchymal cells transition]
Huijun Zhang1, Lei Zhang, Heyong Wang
1Department of Pneumosurgery, Shanghai Pulmonary Hospital, Shanghai, 200433, China.
Background And Objective:
It has been proven that epithelial-mesenchymal transition (EMT) not only correlated with embryonic development but also could promote tumor invasion and metastasis. Transforming growth factor beta-1 (TGF-beta1) has been identified as the main inducer of tumor EMT. The aim of this study was to investigate the effects of TGF-beta1 on EMT and PI3K/AKT signaling pathway in lung adencarcinoma PC9 cells.
Methods:
Cultured PC9 cells were treated with different concentrations of TGF-beta1 for 48 h. The morphological changes were observed under phase-contrast microscopy; EMT relative marker protein changes were assessed by Western blot and immunoflurescence staining. In addition, the expression of AKT and P-AKT were also measured by Western blot.
Results:
The data showed that TGF-beta1 could induce PC9 morphological alteration from epithelial to mesenchymal and upregulate the expression of mesenchymal maker protein Fibronectin. Obviously, the expression of P-AKT was downregulated by TGF-beta1 treatment for 48 h.
Conclusion:
TGF-beta1 might induce EMT of PC9 cells, accompanied by the changes of PI3K/AKT signaling pathway.
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