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Related Concept Videos

Nitric Oxide Signaling Pathway01:28

Nitric Oxide Signaling Pathway

Nitric oxide (NO), an inorganic gas, acts as a potent second messenger in most animal and plant tissues. NO diffuses out of the cells that produce it and enters the neighboring cells to generate a downstream response. NO synthase (NOS) catalyzes NO production by the deamination of the amino acid arginine. There are three isoforms of NOS. Endothelial cells have endothelial NOS (eNOS), nerve and muscle cells have neuronal NOS (nNOS), and macrophages produce inducible NOS (iNOS) upon exposure to...

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A highly selective low-background fluorescent imaging agent for nitric oxide.

Youjun Yang1, Stephanie K Seidlits, Michelle M Adams

  • 1Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, Texas 78712, USA.

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|August 3, 2010
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Summary

Researchers developed NO(550), a novel probe for detecting nitric oxide (NO). This easily synthesized compound offers a rapid, selective, and sensitive method for both intracellular and extracellular NO quantification.

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Area of Science:

  • Chemical sensing
  • Biomedical imaging
  • Molecular probes

Background:

  • Nitric oxide (NO) is a critical signaling molecule.
  • Existing NO detection methods face challenges with selectivity and sensitivity.
  • Development of advanced probes is crucial for biological research.

Purpose of the Study:

  • To introduce a novel sensing mechanism for nitric oxide (NO) detection.
  • To develop an easily synthesized and highly effective NO probe.
  • To evaluate the probe's performance for intra- and extracellular NO quantification.

Main Methods:

  • Synthesis of a novel NO probe, designated NO(550).
  • Characterization of the probe's response to NO, including signal turn-on and linearity.
  • Assessment of selectivity against interfering species and evaluation of cell membrane permeability.
  • In vitro studies using two cell lines to determine cytocompatibility.

Main Results:

  • NO(550) exhibits a rapid, linear, and red-shifted 1500-fold turn-on signal for NO detection.
  • The probe demonstrates excellent selectivity against reactive oxygen/nitrogen species, pH, and common interferents.
  • NO(550) effectively crosses cell membranes but not nuclear membranes, enabling both intra- and extracellular NO measurements.
  • In vitro studies confirmed good cytocompatibility with tested cell lines.

Conclusions:

  • NO(550) represents a superior probe for NO detection due to its high specificity, dark background, facile synthesis, and low pH dependence.
  • The probe's properties make it highly suitable for NO quantification and imaging applications in biological systems.
  • This development offers a valuable tool for researchers studying the roles of NO in various physiological and pathological processes.