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Related Concept Videos

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Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
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Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins
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Transient expression systems for plant-derived biopharmaceuticals.

Tatiana V Komarova1, Selene Baschieri, Marcello Donini

  • 1N.I. Vavilov Institute of General Genetics, Russian Academy of Science and A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Russia.

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Summary

Molecular farming uses transient expression in plants for pharmaceutical proteins. This approach offers speed, low cost, and high yields, making plant-based bioproducts competitive with traditional systems.

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Area of Science:

  • Molecular farming
  • Plant biotechnology
  • Biopharmaceutical production

Background:

  • Transient expression systems for pharmaceutical protein production have been utilized for nearly two decades.
  • Current systems primarily rely on Agrobacterium-mediated delivery and viral expression machinery.
  • These methods are crucial for producing recombinant monoclonal antibodies and vaccines.

Purpose of the Study:

  • To survey the state-of-the-art in transient expression for molecular farming.
  • To highlight the advantages and applications of plant-based viral expression systems.
  • To assess the competitiveness of plant-derived bioproducts against conventional methods.

Main Methods:

  • Utilizing DNA- and RNA-based plant virus genomes for vector design.
  • Employing viral vectors as single- and multicomponent expression systems.
  • Leveraging Agrobacterium-mediated expression for complex protein production.

Main Results:

  • Transient expression systems offer ease of manipulation, speed, low cost, and high protein yields.
  • Agrobacterium-mediated expression facilitates the production of complex, subunit-assembled proteins.
  • Transient expression is preferred for large-scale, contained plant cultivation.

Conclusions:

  • Transient expression in plants is a highly efficient method for biopharmaceutical production.
  • Optimized constructs and delivery methods enhance bioproduct yield and monitoring.
  • Plant-derived bioproducts present a competitive alternative to traditional eukaryotic cell factories.