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Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes
Published on: September 26, 2013
Interleukin-9 as a T helper type 17 cytokine
Elizabeth C Nowak1, Randolph J Noelle
1Department of Microbiology and Immunology, Dartmouth Medical School and Norris Cotton Cancer Center, Lebanon, NH 03756, USA. Elizabeth.C.Nowak@Dartmouth.edu
Immunology
|August 3, 2010
Summary
Interleukin-9 (IL-9) production by CD4 T cells, including Th17 cells, is under investigation. This study explores conditions for generating IL-9 in Th17 cells and their potential role alongside Th9 cells in immune responses.
Area of Science:
- Immunology
- Cell Biology
- T-cell differentiation
Background:
- Interleukin-9 (IL-9) production by CD4 T cells is more widespread than previously understood.
- IL-9 is produced by Th9 cells and other subsets, including Th17 cells.
- The physiological conditions governing IL-9-producing T-cell subsets remain unclear.
Purpose of the Study:
- To investigate the conditions for generating IL-9 in Th17 cells in vitro.
- To explore in vivo conditions where both IL-9 and IL-17 are present.
- To propose the co-existence and interaction of IL-9-producing Th17 cells and Th9 cells in disease states.
Main Methods:
- In vitro culture systems to induce IL-9 production in Th17 cells.
- In vivo analysis of IL-9 and IL-17 co-expression in relevant physiological conditions.
- Literature review and synthesis of existing data on T-cell subset differentiation and cytokine production.
Main Results:
- Defined conditions for in vitro generation of IL-9 in Th17 cells.
- Identified in vivo scenarios where IL-9 and IL-17 co-occur.
- Evidence suggests potential functional interactions between Th17 and Th9 cells.
Conclusions:
- Th17 cells can be induced to produce IL-9 under specific laboratory conditions.
- IL-9 and IL-17 co-production by T cells occurs in vivo.
- IL-9-producing Th17 cells may play a role alongside Th9 cells in autoimmunity, allergy, and infection.
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