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Modeling Spontaneous Metastatic Renal Cell Carcinoma (mRCC) in Mice Following Nephrectomy
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NDRG2 suppresses the proliferation of clear cell renal cell carcinoma cell A-498

Jian-Jun Ma1, Cheng-Gong Liao, Xue Jiang

  • 1Department of Urology, Tangdu Hospital, the Fourth Military Medical University, Xi'an, China.

Journal of Experimental & Clinical Cancer Research : CR
|August 3, 2010
PubMed
Abstract

Insights

N-myc downstream-regulated gene 2 (NDRG2) inhibits clear cell renal cell carcinoma (CCRCC) cell proliferation and induces G1 phase arrest. p53 upregulates NDRG2, suggesting NDRG2 acts as a tumor suppressor in CCRCC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • N-myc downstream-regulated gene 2 (NDRG2) expression is decreased in clear cell renal cell carcinoma (CCRCC).
  • The specific role of NDRG2 in CCRCC progression remains largely undescribed.

Purpose of the Study:

  • To investigate the functional role of NDRG2 in CCRCC.
  • To determine the effect of NDRG2 re-expression on CCRCC cell proliferation and cell cycle.
  • To explore the relationship between NDRG2 and p53 in CCRCC.

Main Methods:

  • NDRG2 recombinant adenovirus plasmid construction.
  • Assessment of cell proliferation using MTT assay.
  • Flow cytometry and Western blot analysis for cell cycle and protein expression.

Main Results:

  • Re-expression of NDRG2 in CCRCC cells significantly decreased proliferation rate.
  • NDRG2 expression led to G1 phase arrest (62.08%) compared to control cells (54.39%).
  • NDRG2 upregulation reduced cyclin D1 and cyclin E, increased apoptosis, and was positively regulated by p53.

Conclusions:

  • NDRG2 inhibits renal carcinoma cell proliferation and induces G1 phase arrest.
  • p53 upregulates NDRG2 expression.
  • NDRG2 functions as a tumor suppressor in CCRCC.

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