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Updated: Jun 10, 2026

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
Published on: October 24, 2018
Kinetic study for a stress testing of l,l-ethylenedicysteine by ultra-performance liquid chromatography/tandem mass
Xiaotao Sun1, Jinping Qiao, Lin Zhu
1Key Laboratory of Radiopharmaceuticals, Ministry of Education, College of Chemistry, Beijing Normal University, Beijing 100875, PR China.
Abstract:
This study proposed a stress testing to study oxidative stability and estimate the potential shelf-life of l,l-ethylenedicysteine (l,l-EC) under normal storage temperature condition (20-25 degrees C). l,l-EC was detected as a function of time at four different temperatures by ultra-performance liquid chromatography/tandem mass spectrometry (UPLC/MS/MS). The degradation of l,l-EC followed the first order kinetics, and the temperature-dependent kinetics was well described by the linear Arrhenius equation. The activation energy (E(a)) was calculated, and the shelf-life at 25 and 4 degrees C was predicted. The results are useful for the proper storage and quality evaluation of l,l-EC.

