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In Situ Ca2+ Imaging of the Enteric Nervous System

In Situ Ca²⁺ Imaging of the Enteric Nervous System

Published on: January 29, 2015

Calbindin-immunoreactive cells in the fish enteric nervous system.

Catharina Olsson¹

¹Department of Zoology/Zoophysiology, University of Gothenburg, Box 463, SE 405 30 Gothenburg, Sweden. c.olsson@zool.gu.se

Autonomic Neuroscience : Basic & Clinical

|August 3, 2010

Summary

Calbindin is found in fish gut neurons, differing from mammalian intrinsic primary afferent neurons (IPANs). This study characterizes calbindin-expressing cells in shorthorn sculpin, revealing distinct properties and a potential homogenous subpopulation.

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Area of Science:

Neuroscience
Gastroenterology
Comparative Anatomy

Background:

Calbindin is prevalent in mammalian intrinsic primary afferent neurons (IPANs) of the gut.
Knowledge regarding calbindin and IPANs in fish is limited.
The enteric nervous system (ENS) in teleosts warrants further investigation.

Purpose of the Study:

To investigate calbindin immunoreactivity in the ENS of the shorthorn sculpin (Myoxocephalus scorpius).
To characterize the calbindin-immunoreactive cell population in the fish gut.
To compare fish calbindin-positive neurons with mammalian IPANs.

Main Methods:

Immunohistochemistry to detect calbindin in the enteric nervous system.
Quantification of calbindin-immunoreactive cells in different gut regions.
Analysis of cell morphology, size distribution, and colocalization with serotonin and choline acetyltransferase (ChAT).

Main Results:

Calbindin-immunoreactive cells and fibers were observed throughout the sculpin gut, excluding the cardiac stomach.
Calbindin-positive neurons constituted a significant proportion (59±6%) of the myenteric neurons in the proximal intestine.
Calbindin-positive neurons were smaller than calbindin-negative neurons and largely colocalized with serotonin, but not ChAT.

Conclusions:

The calbindin-immunoreactive cells in the sculpin gut represent a distinct subpopulation of enteric neurons.
These cells exhibit differences in size and neurotransmitter co-expression compared to mammalian IPANs.
Further functional studies are needed to elucidate the role of these calbindin-positive neurons in fish gut physiology.