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Quantitative MAIPA: Comparison of different MAIPA protocols
Mette Kjær Killie1, Wahida Salma, Eirin Bertelsen
1Department of Immunology and Transfusion Medicine, University Hospital of North Norway, 9038 Tromsø, Norway. mette.kjaer.killie@unn.no
Introduction:
In a screening setting, maternal anti-HPA 1a antibody level has been found to be a good prognostic tool to identify newborns at risk for severe NAIT.
Aim:
Identify the optimal MAIPA protocol for quantitation of anti-HPA 1a antibodies.
Materials And Methods:
Plasma were analysed for anti-HPA 1a antibodies using different monoclonal antibodies, lyophilized or fresh platelets and MAIPA protocols.
Results:
The anti-HPA 1a antibody level varied significantly when different monoclonal antibodies were used. However, there was a strong correlation between maternal anti-HPA 1a antibody level and platelet count in the newborn. The sensitivity of the assay depended on the adopted MAIPA protocol.
Conclusion:
Consistent tests results are of importance for the clinical impact of the test.
