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Updated: Jun 10, 2026

Real-time Live Imaging of T-cell Signaling Complex Formation

Real-time Live Imaging of T-cell Signaling Complex Formation

Published on: June 23, 2013

FasL expression in activated T lymphocytes involves HuR-mediated stabilization.

Gillian L Drury¹, Sergio Di Marco, Virginie Dormoy-Raclet

¹Department of Biochemistry, Rosalind and Morris Goodman Cancer Center, Montreal, Quebec H3G 1Y6, Canada.

The Journal of Biological Chemistry

|August 3, 2010

Summary

This study reveals how the RNA-binding protein HuR stabilizes Fas ligand (FasL) messenger RNA (mRNA) in T cells. This posttranscriptional regulation is crucial for controlling lymphocyte proliferation and maintaining immune tolerance.

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Area of Science:

Immunology
Molecular Biology
Cell Biology

Background:

Prolonged immune activation causes lymphocyte hyperproliferation, leading to autoimmune diseases.
Fas ligand (FasL) induces apoptosis in T cells, controlling excessive lymphoproliferation.

Purpose of the Study:

To investigate the posttranscriptional regulation of FasL expression.
To identify molecular mechanisms controlling FasL mRNA stability in activated T cells.

Main Methods:

Sequence analysis of the FasL 3'-untranslated region (UTR) for AU-rich elements (AREs).
In vitro and ex vivo complex formation assays between FasL mRNA and HuR.
Gene knockdown experiments using HEK 293 cells and a GFP reporter construct.

Main Results:

FasL mRNA is stable in activated T cells.
The FasL 3'-UTR contains AREs similar to TNFα mRNA.
HuR binds to FasL mRNA via these AREs.
HuR knockdown inhibits FasL 3'-UTR-mediated reporter gene expression.

Conclusions:

Posttranscriptional regulation by HuR is a novel mechanism modulating FasL expression.
HuR-mediated stabilization of FasL mRNA is critical for immune system function and tolerance.