Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Genotoxic evaluation of Ara-C by multiple parameters.

K D Beaula Helen1, S Subramanyam

  • 1Department of Genetics, Osmania University, Hyderabad, A.P., India.

Mutation Research
|July 1, 1991
PubMed
Summary

Cytosine arabinoside (Ara-C) causes chromosome mutations in both animal models and human cells. The antimetabolite showed higher mutagenic activity in vivo compared to in vitro.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cases of Interest Seen at the Radiological Department of the Erskine Hospital, Madura, during 1941 and 1942.

The Indian medical gazette·2017
Same author

Quantifying the Assembly of Multicomponent Molecular Machines by Single-Molecule Total Internal Reflection Fluorescence Microscopy.

Methods in enzymology·2016
Same author

Differential expression of candidate salivary effector proteins in field collections of Hessian fly, Mayetiola destructor.

Insect molecular biology·2014
Same author

Further cases of interest seen at the Wilson Radiological Department of the Erskine Hospital, Madura.

The Indian medical gazette·2010
Same author

Rare presentation of unruptured sinus of valsalva aneurysm involving all three sinuses.

Indian heart journal·2009
Same author

Tuberculin skin test results in HIV-infected patients in India: implications for latent tuberculosis treatment.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2008

Area of Science:

  • Cytogenetics
  • Molecular Toxicology
  • Pharmacology

Background:

  • Cytosine arabinoside (Ara-C) is an antimetabolite used in chemotherapy.
  • Understanding its cytogenetic effects is crucial for assessing its safety and efficacy.
  • Previous studies have indicated potential genotoxic effects of Ara-C.

Purpose of the Study:

  • To evaluate the cytogenetic effects of cytosine arabinoside (Ara-C) using both in vivo and in vitro test systems.
  • To assess chromosome aberrations, mitotic index, and sister-chromatid exchanges (SCEs).
  • To compare the mutagenic response of Ara-C in different experimental settings.

Main Methods:

  • In vivo assays using bone marrow and testis cells of Swiss albino mice.
  • In vitro assays using human leukocyte cultures.
  • Evaluation of chromosome aberrations, mitotic index, and SCEs.
  • Statistical analysis of quantitative data.

Main Results:

  • Ara-C demonstrated significant chromosome mutational activity in both in vivo and in vitro experiments.
  • Sister-chromatid exchanges (SCEs) data supported the induction of chromosome mutations.
  • A differential mutagenic response was observed, with higher activity in vivo than in vitro.

Conclusions:

  • Cytosine arabinoside (Ara-C) is a clastogen and mutagen.
  • The in vivo test system showed a more pronounced mutagenic effect compared to the in vitro system.
  • Data from chromosome aberrations and SCEs corroborate the mutagenic potential of Ara-C.

Related Experiment Videos