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Simple Polyacrylamide-based Multiwell Stiffness Assay for the Study of Stiffness-dependent Cell Responses
Published on: March 25, 2015
Mechanical regulation of cell function with geometrically modulated elastomeric substrates
Jianping Fu1, Yang-Kao Wang, Michael T Yang
1Department of Bioengineering, University of Pennsylvania, Philadelphia, PA, USA.
Nature Methods
|August 3, 2010
Abstract:
We report the establishment of a library of micromolded elastomeric micropost arrays to modulate substrate rigidity independently of effects on adhesive and other material surface properties. We demonstrated that micropost rigidity impacts cell morphology, focal adhesions, cytoskeletal contractility and stem cell differentiation. Furthermore, early changes in cytoskeletal contractility predicted later stem cell fate decisions in single cells.
