Related Experiment Video

Updated: Jun 10, 2026

Primary Sjogren's Syndrome Associated with Lung Adenocarcinoma: Probing the Potential Common Pathogenic Mechanisms and Experimental Verification
10:21

Primary Sjogren's Syndrome Associated with Lung Adenocarcinoma: Probing the Potential Common Pathogenic Mechanisms and Experimental Verification

Published on: September 20, 2024

Functional interaction between Env oncogene from Jaagsiekte sheep retrovirus and tumor suppressor Sprouty2

Ebenezer Chitra1, Yi-Wen Lin, Fabian Davamani

  • 1Vaccine R&D Center, National Health Research Institutes, 35, Keyan Road, Zhunan, Miaoli County 350, Taiwan.

Retrovirology
|August 4, 2010
PubMed
Abstract

Insights

Jaagsiekte sheep retrovirus (JSRV) Envelope (Env) protein transforms lung cells, altering proliferation and migration. Sprouty2 acts as a tumor suppressor, potentially offering a therapeutic target against JSRV-induced cancers.

Area of Science:

  • Oncology
  • Virology
  • Molecular Biology

Background:

  • Jaagsiekte sheep retrovirus (JSRV), a type D retrovirus, can transform cells in vitro and in vivo.
  • The JSRV Envelope (Env) gene's oncogenic transformation mechanism requires further elucidation, with host factors playing a key regulatory role.

Purpose of the Study:

  • To investigate the role of JSRV Env in cell transformation and identify associated host cell factors.
  • To explore the functional relationship between JSRV Env and Sprouty2 in oncogenesis.

Main Methods:

  • Transformation of lung adenocarcinoma and normal lung epithelial cell lines with JSRV Env.
  • Analysis of cellular signaling pathways (ERK, PI3K-Akt, STAT3) and Sprouty2 expression.
  • Overexpression of Sprouty2 and its mutants in target cells.

Main Results:

  • JSRV Env-mediated transformation induced proliferation and anchorage-independent growth but abrogated migration in cancer cells.
  • Sprouty2 expression was induced by Env, correlating with transformation effects and acting as a tumor suppressor by inhibiting migration and tumor formation.
  • Sprouty2's tumor-suppressive function was confirmed by its ability to confer resistance to Env-mediated transformation.

Conclusions:

  • JSRV Env and Sprouty2 share a functional relationship, likely via common signaling pathways.
  • Sprouty2 acts as a tumor suppressor in JSRV-induced cell transformation.
  • Sprouty2 holds potential as a therapeutic agent against JSRV-associated cancers.

More Related Videos

A Syngeneic Orthotopic Osteosarcoma Sprague Dawley Rat Model with Amputation to Control Metastasis Rate07:31

A Syngeneic Orthotopic Osteosarcoma Sprague Dawley Rat Model with Amputation to Control Metastasis Rate

Published on: May 3, 2021

Related Concept Videos

Mechanisms of Retrovirus-induced Cancers01:51

Mechanisms of Retrovirus-induced Cancers

Retroviruses are RNA viruses that have been shown to cause cancers in diverse species, including chickens, mice, cats, and monkeys. The RNA genomes of these viruses are first reverse-transcribed into single and then double-stranded DNA (dsDNA) copies. This dsDNA called proviral DNA then integrates into the host genome. Subsequently, the host cell transcribes the proviral DNA in concert with the chromosomal DNA. This leads to the production of viral RNA and proteins that assemble at the host...
Mechanisms of Retrovirus-induced Cancers01:51

Mechanisms of Retrovirus-induced Cancers

Retroviruses are RNA viruses that have been shown to cause cancers in diverse species, including chickens, mice, cats, and monkeys. The RNA genomes of these viruses are first reverse-transcribed into single and then double-stranded DNA (dsDNA) copies. This dsDNA called proviral DNA then integrates into the host genome. Subsequently, the host cell transcribes the proviral DNA in concert with the chromosomal DNA. This leads to the production of viral RNA and proteins that assemble at the host...
Rous Sarcoma Virus (RSV) and Cancer01:03

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...
Rous Sarcoma Virus (RSV) and Cancer01:03

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...