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Related Concept Videos

Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...

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Reply to Byker Shanks et al. Measurement of Fruit and Vegetable Intake Incorporating a Diversity, Equity, and Inclusion Lens. Comment on "Di Noia, J.; Gellermann, W. Use of the Spectroscopy-Based Veggie Meter<sup>®</sup> to Objectively Assess Fruit and Vegetable Intake in Low-Income Adults. <i>Nutrients</i> 2021, <i>13</i>, 2270".

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Related Experiment Video

Updated: Jun 10, 2026

Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum through a Chemometric Approach
09:32

Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum through a Chemometric Approach

Published on: September 26, 2019

Validation model for Raman based skin carotenoid detection.

Igor V Ermakov1, Werner Gellermann

  • 1Department of Physics and Astronomy, University of Utah, Salt Lake City, UT 84112, USA.

Archives of Biochemistry and Biophysics
|August 4, 2010
PubMed
Summary

Resonance Raman spectroscopy (RRS) offers a non-invasive way to measure skin carotenoids, important antioxidants and dietary biomarkers. This study validates RRS against chromatography, showing excellent correlation for accurate in vivo carotenoid assessment.

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Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
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Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy

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Last Updated: Jun 10, 2026

Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum through a Chemometric Approach
09:32

Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum through a Chemometric Approach

Published on: September 26, 2019

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
15:04

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy

Published on: May 18, 2011

Area of Science:

  • Biophotonics and Spectroscopy
  • Nutritional Biochemistry
  • Dermatology

Background:

  • Carotenoids are vital antioxidants and photoprotective compounds found in human tissues.
  • Dietary intake of fruits and vegetables influences skin carotenoid levels, making them potential biomarkers.
  • Non-invasive optical methods are needed to quantify carotenoids in vivo, overcoming limitations of traditional chromatography.

Purpose of the Study:

  • To validate in vivo resonance Raman spectroscopy (RRS) for quantifying skin carotenoids.
  • To compare RRS measurements with gold-standard high-performance liquid chromatography (HPLC) analysis.
  • To establish RRS as a reliable method for assessing carotenoid status and dietary intake.

Main Methods:

  • Investigated in vivo RRS measurements of carotenoids in heel skin.
  • Correlated RRS data with subsequent ex vivo HPLC analysis of carotenoid concentrations.
  • Utilized heel skin due to stratum corneum thickness and minimal interfering chromophores.

Main Results:

  • Achieved an excellent correlation (R=0.95) between in vivo RRS and HPLC measurements.
  • Demonstrated that heel skin is a suitable model site for RRS validation studies.
  • Confirmed the accuracy of RRS for quantitative carotenoid assessment in human skin.

Conclusions:

  • Resonance Raman spectroscopy is a valid, non-invasive, and objective method for in vivo carotenoid quantification in human skin.
  • RRS can serve as a reliable tool for monitoring carotenoid status and indirectly assessing fruit and vegetable consumption.
  • This validated method supports future large-scale studies on carotenoids' health benefits and dietary intake.