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Related Experiment Video

Updated: Jun 10, 2026

Mosaic Zebrafish Transgenesis for Evaluating Enhancer Sequences
07:23

Mosaic Zebrafish Transgenesis for Evaluating Enhancer Sequences

Published on: July 16, 2010

Mosaic zebrafish transgenesis for evaluating enhancer sequences.

Erika Kague1, Christopher Weber, Shannon Fisher

  • 1Department of Cell and Developmental Biology, University of Pennsylvania-School of Medicine, PA, USA.

Journal of Visualized Experiments : Jove
|August 4, 2010
PubMed
Summary
This summary is machine-generated.

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Scientists developed a new zebrafish transgenesis method to functionally test non-coding DNA sequences for gene regulation. This approach efficiently identifies cis-regulatory elements, aiding in understanding genome function.

Area of Science:

  • Genomics
  • Developmental Biology
  • Molecular Biology

Background:

  • The human genome project revealed numerous non-coding sequences with unknown functions.
  • Identifying cis-regulatory elements that control gene transcription from DNA sequence remains a significant challenge.
  • Current methods for evaluating cis-regulatory elements have limitations in speed, cost, or biological relevance.

Purpose of the Study:

  • To develop an efficient and broadly applicable method for functionally evaluating potential cis-regulatory elements.
  • To overcome limitations of existing cell-culture or transgenic mouse models for studying gene regulation.
  • To enable rapid assessment of human non-coding sequences using a vertebrate model system.

Main Methods:

  • Selected potential cis-regulatory elements based on DNA sequence conservation across species.

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The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach
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The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach

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Related Experiment Videos

Last Updated: Jun 10, 2026

Mosaic Zebrafish Transgenesis for Evaluating Enhancer Sequences
07:23

Mosaic Zebrafish Transgenesis for Evaluating Enhancer Sequences

Published on: July 16, 2010

Mosaic Zebrafish Transgenesis for Functional Genomic Analysis of Candidate Cooperative Genes in Tumor Pathogenesis
09:45

Mosaic Zebrafish Transgenesis for Functional Genomic Analysis of Candidate Cooperative Genes in Tumor Pathogenesis

Published on: March 31, 2015

The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach
10:00

The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach

Published on: November 30, 2022

  • Developed a protocol for cloning candidate sequences.
  • Utilized zebrafish transgenesis by microinjecting DNA into embryos to assess regulatory function in vivo.

    • Main Results:

    • Demonstrated the utility of zebrafish transgenesis for functional evaluation of cis-regulatory elements.
    • The method provides spatial and temporal gene regulation information relevant to development.
    • This approach is faster and more cost-effective than transgenic mouse studies.

    Conclusions:

    • Zebrafish transgenesis offers an efficient platform for identifying and characterizing cis-regulatory elements.
    • This protocol facilitates the functional annotation of non-coding genomic sequences.
    • The method is applicable to sequences from the human genome, advancing our understanding of gene regulation.