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Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)
Published on: March 24, 2016
Mass spectrometry imaging of small molecules using matrix-enhanced surface-assisted laser desorption/ionization mass
Qiang Liu1, Yongsheng Xiao, Lin He
1Department of Pathology & Lab Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Abstract:
Surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) uses inorganic particles or porous surfaces as the energy-mediating means to promote desorption and ionization of low-mass analytes of interest. With good stability during laser ablation, SALDI substrates exhibit reduced background in the low-mass region that is often crowded in conventional matrix-assisted laser desorption/ionization (MALDI) due to matrix fragmentation; a benefit renders SALDI-MS attractive in imaging low-mass species. Practical application of SALDI-MS, however, is hindered by its unsatisfied detection sensitivity for most compounds. With aims of improving MS imaging resolution and sensitivity of low-mass species, we describe an experimental protocol using a hybrid ionization method, termed matrix-enhanced SALDI (ME-SALDI), to detect crucial low-mass species with their spatial distribution in mouse brain tissue.
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