Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical pregnancy in Turner syndrome following re-implantation of cryopreserved ovarian cortex.

Journal of assisted reproduction and genetics·2023
Same author

What can stem cell technology offer to IVF patients?

BJOG : an international journal of obstetrics and gynaecology·2019
Same author

The existence and potential of germline stem cells in the adult mammalian ovary.

Climacteric : the journal of the International Menopause Society·2019
Same author

Metaphase II oocytes from human unilaminar follicles grown in a multi-step culture system.

Molecular human reproduction·2018
Same author

Maternal and early life nutrition and physical activity: setting the research and intervention agenda for addressing the double burden of malnutrition in South African children.

Global health action·2017
Same author

Non-growing follicle density is increased following adriamycin, bleomycin, vinblastine and dacarbazine (ABVD) chemotherapy in the adult human ovary.

Human reproduction (Oxford, England)·2016

Related Experiment Video

Updated: Jun 10, 2026

Isolation of Small Preantral Follicles from the Bovine Ovary Using a Combination of Fragmentation, Homogenization, and Serial Filtration
09:11

Isolation of Small Preantral Follicles from the Bovine Ovary Using a Combination of Fragmentation, Homogenization, and Serial Filtration

Published on: September 27, 2022

Method for isolating preantral follicles from mare ovaries

E E Telfer1, E D Watson

  • 1Institute of Ecology and Resource Management, Division of Biological Sciences, University of Edinburgh, School of Agriculture Building, West Mains Road, Edinburgh EH9 3JG, UK.

Journal of Reproduction and Fertility. Supplement
|August 5, 2010
PubMed
Summary

Collagenase effectively isolates equine preantral follicles from ovaries. Optimal results require specific concentrations and incubation times to maintain follicle morphology for further research.

More Related Videos

Extraction, Labeling, and Purification of Lineage-Specific Cells from Human Antral Follicles
06:36

Extraction, Labeling, and Purification of Lineage-Specific Cells from Human Antral Follicles

Published on: November 30, 2022

Isolation and Characterization of Mouse Antral Oocytes Based on Nucleolar Chromatin Organization
07:16

Isolation and Characterization of Mouse Antral Oocytes Based on Nucleolar Chromatin Organization

Published on: January 7, 2016

Related Experiment Videos

Last Updated: Jun 10, 2026

Isolation of Small Preantral Follicles from the Bovine Ovary Using a Combination of Fragmentation, Homogenization, and Serial Filtration
09:11

Isolation of Small Preantral Follicles from the Bovine Ovary Using a Combination of Fragmentation, Homogenization, and Serial Filtration

Published on: September 27, 2022

Extraction, Labeling, and Purification of Lineage-Specific Cells from Human Antral Follicles
06:36

Extraction, Labeling, and Purification of Lineage-Specific Cells from Human Antral Follicles

Published on: November 30, 2022

Isolation and Characterization of Mouse Antral Oocytes Based on Nucleolar Chromatin Organization
07:16

Isolation and Characterization of Mouse Antral Oocytes Based on Nucleolar Chromatin Organization

Published on: January 7, 2016

Area of Science:

  • Reproductive Biology
  • Veterinary Science
  • Ovarian Tissue Engineering

Background:

  • Preantral follicles are crucial for mammalian reproduction and fertility preservation.
  • Efficient isolation methods are needed for studying equine oocyte development and assisted reproductive technologies.

Purpose of the Study:

  • To evaluate collagenase treatment for isolating equine preantral follicles.
  • To assess the impact of collagenase on the morphology of isolated follicles.

Main Methods:

  • Equine ovaries were minced and incubated with varying concentrations of collagenase (1, 3, or 5 mg/ml) at 37°C.
  • Follicles were isolated using sequential filtration (50-300 µm pores).
  • Histological analysis and light microscopy were used to assess follicle morphology and count.

Main Results:

  • Optimal isolation of intact preantral follicles (90-150 µm diameter) was achieved with 3 or 5 mg/ml collagenase after 60-90 minutes.
  • Higher collagenase concentrations and longer incubation times (up to 2 hours) did not significantly increase follicle yield but decreased quality.
  • No intact follicles were recovered after 2 hours of incubation.

Conclusions:

  • Collagenase treatment is a viable method for isolating equine preantral follicles.
  • Appropriate collagenase concentration and incubation duration are critical to preserve follicle morphology.
  • Further studies are needed to determine oocyte quality and follicle viability post-isolation.