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Updated: Jun 10, 2026

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors

Published on: September 20, 2016

Molecular pathology--the future?

G G A Hutchins¹, H I Grabsch

¹Section of Pathology and Tumour Biology, Leeds Institute of Molecular Medicine, University of Leeds, UK.

The Surgeon : Journal of the Royal Colleges of Surgeons of Edinburgh and Ireland

|August 5, 2010

Summary

Histopathology, the microscopic examination of tissues, is evolving beyond morphology. Molecular characterization is crucial for diagnosing and managing complex diseases, driving the need for advanced molecular techniques in pathology.

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Area of Science:

Pathology
Molecular Biology
Oncology

Background:

Histopathological assessment via light microscopy is the gold standard for disease diagnosis.
Morphological analysis alone is insufficient for complex diseases with altered molecular characteristics.
Molecular characterization is increasingly vital for accurate disease diagnosis and therapeutic strategies.

Purpose of the Study:

To review current molecular techniques used in histopathology.
To explore novel high-throughput technologies for future histopathological practice.
To highlight the integration of molecular data into disease diagnosis and management.

Main Methods:

Review of existing literature on molecular techniques in histopathology.
Discussion of immunohistochemistry and fluorescent in situ hybridization.
Exploration of emerging high-throughput and array-based technologies.

Main Results:

Current molecular techniques like immunohistochemistry and FISH are widely used.
The demand for detailed molecular classification of diseases is rapidly increasing.
Novel high-throughput technologies are poised to impact future histopathological practice.

Conclusions:

Histopathology must integrate molecular characterization for comprehensive disease diagnosis.
Advanced molecular techniques are essential for personalized medicine and targeted therapies.
The future of histopathology lies in combining morphological and molecular insights for improved patient outcomes.