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Measuring Composition of CD95 Death-Inducing Signaling Complex and Processing of Procaspase-8 in this Complex
Published on: August 2, 2021
CD95 is not functional in human erythrocytes
D Sagan1, N Jermnim, Orathai Tangvarasittichai
1Faculty of Allied Health Sciences, Naresuan University, Phitsanulok, Thailand.
International Journal of Laboratory Hematology
|August 5, 2010
Summary
The CD95 death receptor pathway is not functional in human red blood cells. Activation of CD95 does not induce phosphatidylserine exposure, a key marker of eryptosis in erythrocytes.
Area of Science:
- Hematology
- Cell Biology
- Immunology
Background:
- The CD95 pathway induces apoptosis in nucleated cells and is crucial for blood cell development.
- Red blood cells express CD95, but its function in these anucleated cells is unclear.
- Eryptosis, a form of red blood cell death, shares similarities with apoptosis and is triggered by oxidative stress or energy depletion.
Purpose of the Study:
- To investigate the functional role of the CD95 death receptor pathway in human erythrocytes.
- To determine if CD95 activation can induce phosphatidylserine (PS) exposure in red blood cells.
Main Methods:
- Purified human erythrocytes were treated with CH11, an antibody targeting CD95.
- Annexin V-FITC was used to detect and quantify phosphatidylserine exposure on the erythrocyte surface.
Main Results:
- Treatment of erythrocytes with CH11 did not result in a significant increase in phosphatidylserine-positive cells.
- These findings indicate that CD95 activation does not trigger PS exposure in red blood cells.
Conclusions:
- The CD95 death receptor system is not functional in mature human red blood cells.
- Activation of CD95 in erythrocytes does not lead to the characteristic phosphatidylserine exposure associated with eryptosis or apoptosis.
