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Updated: Jun 10, 2026

Crystallization of Membrane Proteins in Lipidic Mesophases
11:53

Crystallization of Membrane Proteins in Lipidic Mesophases

Published on: March 28, 2011

Crystallizing transmembrane peptides in lipidic mesophases.

Nicole Höfer1, David Aragão, Martin Caffrey

  • 1Membrane Structural and Functional Biology Group, School of Biochemistry and Immunology, and School of Medicine, Trinity College, Dublin, Ireland.

Biophysical Journal
|August 5, 2010
PubMed
Summary

The in meso method enables high-resolution membrane protein structure determination, even for small proteins. This technique, using lipidic mesophases, successfully crystallized a small peptide, expanding its applicability.

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Crystallization of Membrane Proteins in Lipidic Mesophases
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Published on: March 28, 2011

Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
22:00

Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases

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From Constructs to Crystals &#8211; Towards Structure Determination of &#946;-barrel Outer Membrane Proteins
09:55

From Constructs to Crystals – Towards Structure Determination of β-barrel Outer Membrane Proteins

Published on: July 4, 2016

Area of Science:

  • Structural biology
  • Membrane protein crystallography
  • Biophysics

Background:

  • Crystallization in lipidic mesophases (in meso method) aids membrane protein structure determination.
  • The in meso method's applicability to small proteins (<=4 transmembrane crossings) was previously questioned.

Purpose of the Study:

  • To test if the in meso method can crystallize small membrane proteins.
  • To determine the structure of linear gramicidin using this technique.

Main Methods:

  • Crystallization of linear gramicidin in lipidic mesophases.
  • High-resolution structure determination of the crystallized peptide.

Main Results:

  • Linear gramicidin, a transmembrane pentadecapeptide, formed structure-grade crystals using the in meso method.
  • The inherent flexibility of the mesophase facilitated crystallogenesis of this small protein target.

Conclusions:

  • The in meso method is viable for high-resolution structure determination of integral membrane peptides.
  • This technique can be applied to small membrane proteins, including those encoded in the human genome.