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Updated: Jun 10, 2026
Isolation and Characterization of Tumor-initiating Cells from Sarcoma Patient-derived Xenografts
Published on: June 13, 2019
Heterogeneity of radiation- and TNF-alpha-induced programmed cell death in carcinoma, sarcoma and lymphoma cell lines
Tobias Bölling1, Astrid Kolkmeyer, Burkhard Greve
1Department of Radiotherapy, University Hospital Münster, Albert-Schweitzer-Str. 33, 48149 Münster, Germany. Tobias.Boelling@uni-muenster.de
Aim:
To characterize the interaction of tumour necrosis factor alpha (TNF-alpha) and ionising radiation in six sarcoma, lymphoma and carcinoma cell lines.
Materials And Methods:
Cells were characterized regarding annexin V/propiduim iodine affinity and caspase-3 status after application of TNF-alpha, radiation, or combined treatment.
Results:
Three cell lines showed similar results with additive effects of TNF-alpha and radiation in both assays. The other three cell lines significantly differed regarding the detection of apoptotic cells between treatment conditions (radiation and/or TNF application) and between the two apoptosis assays.
Conclusion:
The interaction between TNF-alpha and radiation differs between tumour entities and cannot be described by using only one parameter.
