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Related Experiment Video

Updated: Jun 10, 2026

The 3D Culturing of Organoids from Murine Intestinal Crypts and a Single Stem Cell for Organoid Research

The 3D Culturing of Organoids from Murine Intestinal Crypts and a Single Stem Cell for Organoid Research

Published on: April 7, 2023

Intestinal stem cells.

¹Department of Internal Medicine, Division of Digestive Diseases, University of Oklahoma Health Sciences Center, 975 NE 10th Street, SL Young BRC West 1268B, Oklahoma City, OK 73104, USA. Shahid-Umar@ouhsc.edu

Current Gastroenterology Reports

|August 5, 2010

Summary

This summary is machine-generated.

Intestinal stem cells (ISCs) fuel gut epithelial self-renewal. Their precise location and the signals governing their transition to progenitor cells remain unclear, suggesting distinct ISC regions and activation states.

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Generation, Maintenance, and Characterization of Human Pluripotent Stem Cell-derived Intestinal and Colonic Organoids

Generation, Maintenance, and Characterization of Human Pluripotent Stem Cell-derived Intestinal and Colonic Organoids

Published on: July 9, 2021

Area of Science:

Gastroenterology
Stem Cell Biology
Epithelial Biology

Background:

Intestinal self-renewal relies on undifferentiated intestinal stem cells (ISCs).
ISCs differentiate into various cell types crucial for gut function.
The signals regulating ISC self-renewal and the transition to progenitor cells are largely unknown.

Purpose of the Study:

To investigate the poorly understood cellular signals governing intestinal stem cell (ISC) self-renewal.
To identify the factors mediating the transition of ISCs to progenitor cells in the gut.
To explore the potential existence of distinct ISC regions within the intestinal crypt.

Main Methods:

Review of recent studies on ISC localization and markers.
Analysis of proposed ISC markers like Lgr-5, DCAMKL-1, and Bmi-1.
Hypothetical modeling of ISC activation states and their regulatory mechanisms.

Main Results:

Recent research suggests ISCs may reside at the crypt base (e.g., Lgr-5+ve cells) or near position 4 (e.g., DCAMKL-1 or Bmi-1+ve cells).
This indicates the possibility of distinct stem cell niches within the intestinal crypts.
ISC activation state may determine the regulation of self-renewal in the intestinal tract.

Conclusions:

Distinct stem cell regions might exist within intestinal crypts.
The activation state of intestinal stem cells could be a key factor in regulating gut self-renewal.
Further research is needed to elucidate the precise mechanisms and locations of ISC regulation.