Related Experiment Video

Updated: Jun 10, 2026

Determining the Reactivity and Titre of Serum using a Haemagglutination Assay
05:59

Determining the Reactivity and Titre of Serum using a Haemagglutination Assay

Published on: January 29, 2010

[The effect of blood serum polyreactive immunoglobulins on serum antibody affinity determination]

S A Bobrovnik, M A Demchenko, S V Komisarenko

    Ukrains'Kyi Biokhimichnyi Zhurnal (1999 )
    |August 6, 2010
    PubMed

    The presence of polyreactive immunoglobulins in sera may substantially influence on the accuracy of antibody affinity determination. In order to obtain precise values of antibody affinity one should apply one of two following ways. First, one should block polyreactive immunoglobulins with high concentration of Twin 20 and high concentration of any antigen which does not interact with studying antibody. After this antibody affinity may be determined by traditional methods. Another way is the application of the method suggested by us earlier, which allow determining affinity of two antibodies in a mixture and the relation of their concentrations.

    More Related Videos

    Rapid Determination of Antibody-Antigen Affinity by Mass Photometry10:17

    Rapid Determination of Antibody-Antigen Affinity by Mass Photometry

    Published on: February 8, 2021

    Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms15:27

    Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms

    Published on: April 17, 2017

    Related Concept Videos

    Affinity and Avidity01:41

    Affinity and Avidity

    Overview
    Affinity Chromatography01:03

    Affinity Chromatography

    Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
    Blood Typing01:10

    Blood Typing

    Understanding an individual's blood group is a critical component of transfusion medicine. It ensures compatibility in blood transfusions, organ transplants, and even during pregnancy. Determining these blood groups involves the ABO and Rh blood typing systems, utilizing specific antigens and corresponding anti-sera to identify an individual's blood type.
    Antigens are protein molecules that reside on the surface of red blood cells (RBCs). The ABO and Rh blood typing systems target antigens A,...