Related Experiment Video

Updated: Jun 10, 2026

Genome-wide RNAi Screening to Identify Host Factors That Modulate Oncolytic Virus Therapy
08:51

Genome-wide RNAi Screening to Identify Host Factors That Modulate Oncolytic Virus Therapy

Published on: April 3, 2018

Oncolytic-adenovirus-expressed RNA interference for cancer therapy

Dong-Sheng Pei1, Jie-Hui Di, Fei-Fei Chen

  • 1Xuzhou Medical College, Laboratory of Biological Cancer Therapy, 84 West Huai-hai Road, Xuzhou, Jiangsu 221002, PR China. jnzheng@xzmc.edu.cn

Expert Opinion on Biological Therapy
|August 6, 2010
PubMed
Abstract

Insights

Oncolytic adenovirus effectively delivers small interfering RNA (siRNA) to cancer cells, enhancing antitumor responses. This combined approach leverages gene knockdown and viral oncolysis for potent cancer treatment.

Area of Science:

  • Oncology
  • Gene Therapy
  • Virology

Background:

  • RNA interference (RNAi) shows promise for cancer therapy.
  • Efficient delivery of small interfering RNA (siRNA) to cancer cells is crucial due to delivery challenges and short siRNA half-life.
  • Oncolytic adenovirus presents an effective platform for siRNA delivery, offering high transfection efficiency and cancer-selective replication.

Purpose of the Study:

  • To review the synergistic antitumor effects of combining oncolytic adenovirus and siRNA.
  • To discuss advancements in oncolytic adenovirus-expressed siRNA technology.

Main Methods:

  • Focus on the synergy between oncolytic adenovirus and siRNA in antitumor responses.
  • Review of recent progress in siRNA expression by oncolytic adenoviruses.

Main Results:

  • Oncolytic adenovirus-mediated siRNA delivery significantly enhances antitumor effects.
  • The enhanced efficacy results from combined gene knockdown by siRNA and viral oncolysis.

Conclusions:

  • Oncolytic adenovirus-based siRNA delivery is a potent and targeted strategy for cancer treatment.
  • This approach holds significant promise for developing novel human cancer therapies.

More Related Videos

DNA Vector-based RNA Interference to Study Gene Function in Cancer13:10

DNA Vector-based RNA Interference to Study Gene Function in Cancer

Published on: June 4, 2012

Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo12:42

Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo

Published on: January 7, 2019

Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
Rous Sarcoma Virus (RSV) and Cancer01:03

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...
Rous Sarcoma Virus (RSV) and Cancer01:03

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...