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Synthesis, Cellular Delivery and In vivo Application of Dendrimer-based pH Sensors
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Multicomponent polymeric micelles based on polyaspartamide as tunable fluorescent pH-window biosensors.

Yuri Antonio Diaz-Fernandez1, Elisa Mottini, Luca Pasotti

  • 1Dipartimento di Chimica Generale, Università di Pavia, viale Taramelli 12, 27100 Pavia, Italy.

Biosensors & Bioelectronics
|August 6, 2010
PubMed
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Polyaspartamide polymer micelles form a biocompatible sensor. This novel micellar device acts as an "off-on-off" fluorescence sensor for pH, suitable for in vivo applications.

Area of Science:

  • Polymer Chemistry
  • Materials Science
  • Biotechnology

Background:

  • Polyaspartamide polymers offer tunable properties for advanced applications.
  • Biocompatible micelles are crucial for drug delivery and sensing.
  • Developing efficient pH sensors is vital for biological and medical research.

Purpose of the Study:

  • To synthesize and characterize PHEA-PEG(5000)-C(16) polyaspartamide polymer micelles.
  • To investigate the physicochemical properties of the micellar core.
  • To develop a novel "off-on-off" fluorescence pH sensor based on these micelles.

Main Methods:

  • Potentiometric titrations to determine protonation and acidity constants.
  • Fluorescent probes (pyrene) to study micellar core viscosity and polarity.

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  • Investigation of sensor response with varying pH and cosurfactants.
  • Dialysis experiments to assess component retention.

    • Main Results:

    • PHEA-PEG(5000)-C(16) forms biocompatible micelles with a low critical aggregation concentration (CAC).
    • Micellar core properties are comparable to organic solvents and other surfactant micelles.
    • The micellar device functions as a unique "off-on-off" fluorescence pH sensor, with the "on" state covering the physiological pH range (6-8).
    • Sensor's pH sensitivity can be tuned by charged cosurfactants.
    • Efficient retention of all components, including cosurfactants, was demonstrated.

    Conclusions:

    • PHEA-PEG(5000)-C(16) micelles provide a stable and tunable platform for fluorescence sensing.
    • The developed micellar sensor exhibits potential for in vivo applications due to its biocompatibility and retention capabilities.
    • This work presents a promising tool for real-time pH monitoring in biological systems.