Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 10, 2026

Generation and Grafting of Tissue-engineered Vessels in a Mouse Model
13:04

Generation and Grafting of Tissue-engineered Vessels in a Mouse Model

Published on: March 18, 2015

Pericyte-based human tissue engineered vascular grafts.

Wei He1, Alejandro Nieponice, Lorenzo Soletti

  • 1Department of Surgery, University of Pittsburgh, USA.

Biomaterials
|August 6, 2010
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Amine-Reactive Augmentation of Silk Fibroin Mats for Increasing Cargo Retention Capabilities.

Journal of functional biomaterials·2026
Same author

[Surgical Procedures in Gastroesophageal Reflux Disease].

Acta gastroenterologica Latinoamericana·2026
Same author

"Attractive" Treatment for Abdominal Aortic Aneurysm Repair: Magnetic Localization of Silk-Iron Packaged Extracellular Vesicles.

Journal of functional biomaterials·2025
Same author

Sex-specific machine learning classification models improve outcome prediction for abdominal aortic aneurysms.

Biology of sex differences·2025
Same author

Mesenchymal progenitor cells in perivascular niches: forerunners of mesenchymal stem cells and players in tissue scarring and regeneration.

Vascular pharmacology·2025
Same author

Wall Tensile Stress Maps of Human Aneurysmal Aorta Demonstrate a High Biaxiality Ratio Corresponds with Wall Tissue Microstructure and Local Oxidative Stress Response Distinctly for Bicuspid and Tricuspid Aortic Valve Patients.

Annals of biomedical engineering·2025

Pericytes efficiently seeded onto vascular scaffolds significantly improve graft patency. These pericyte-based tissue engineered vascular grafts (TEVGs) demonstrate successful tissue remodeling and arterial conduit function in vivo.

Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Vascular Surgery

Background:

  • Tissue engineered vascular grafts (TEVGs) require optimal cell sources and delivery systems for small-diameter applications.
  • Pericytes, possessing mesenchymal stem cell characteristics, offer a promising cell source due to their availability and multipotentiality.

Purpose of the Study:

  • To efficiently incorporate pericytes into biodegradable scaffolds for TEVG fabrication.
  • To evaluate the in vivo efficacy of pericyte-seeded scaffolds as aortic interposition grafts.

Main Methods:

  • Bi-layered poly(ester-urethane)urea scaffolds were rapidly bulk seeded with pericytes (>90% efficiency) using a rotational vacuum device.
  • Seeded scaffolds underwent dynamic culture before implantation into Lewis rats for 8 weeks.

More Related Videos

Implantation of Tissue-Engineered Vascular Graft in Mouse Carotid Artery via Cuff Technique
07:13

Implantation of Tissue-Engineered Vascular Graft in Mouse Carotid Artery via Cuff Technique

Published on: May 2, 2025

Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor
11:22

Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor

Published on: June 14, 2011

Related Experiment Videos

Last Updated: Jun 10, 2026

Generation and Grafting of Tissue-engineered Vessels in a Mouse Model
13:04

Generation and Grafting of Tissue-engineered Vessels in a Mouse Model

Published on: March 18, 2015

Implantation of Tissue-Engineered Vascular Graft in Mouse Carotid Artery via Cuff Technique
07:13

Implantation of Tissue-Engineered Vascular Graft in Mouse Carotid Artery via Cuff Technique

Published on: May 2, 2025

Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor
11:22

Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor

Published on: June 14, 2011

  • Graft patency, tissue remodeling, cellular infiltration, and phenotypic markers were assessed.

    • Main Results:

    • Pericytes evenly populated scaffolds and maintained their phenotype after dynamic culture.
    • Pericyte-seeded TEVGs exhibited a significantly higher patency rate (100%) compared to unseeded controls (38%).
    • Implanted grafts showed extensive remodeling with collagen, elastin, smooth muscle actin, calponin, and von Willebrand factor expression.

    Conclusions:

    • A pericyte-based TEVG approach is feasible, utilizing efficient seeding and dynamic culture.
    • Pericytes play a crucial role in maintaining the patency and promoting tissue regeneration in vascular grafts.
    • Pericyte-seeded TEVGs show potential as functional arterial conduits.